Grain protein content (GPC) in wheat has been a major trait of interest for breeders since it has enormousend use potential. In the present study, marker-assisted backcrossing (MABC) was successfully used toimprove GPC in wheat cultivar HUW468. The genotype Glu269 was used as the donor parent for intro-gression of the gene Gpc-B1 that confers high GPC. In a segregating population, SSR marker Xu…