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Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa)

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Phosphorus (P) is crucial nutrient element for crop growth and development. However, the
network pathway regulating homeostasis of phosphate (Pi) in crops has many molecular
breeding unknowns. Here, we report that an auxin response factor, OsARF12, functions in Pi
homeostasis.

Measurement of element content, quantitative reverse transcription polymerase chain reaction
analysis and acid phosphatases (APases) activity assay showed that the osarf12 mutant
and osarf12/25 double mutant with P-intoxicated phenotypes had higher P concentrations,
up-regulation of the Pi transporter encoding genes and increased APase activity under
Pi-sufficient/-deficient (+Pi/Pi, 0.32/0mM NaH2PO4) conditions.

Transcript analysis revealed that Pi-responsive genes – Phosphate starvation (OsIPS)1 and
OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3
MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1) – were
more abundant in the osarf12 and osarf12/25 mutants under +Pi/Pi conditions. Knockout
of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream
components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPXMFS2.
Results from –Pi/1-naphthylphthalamic acid (NPA) treatments, and auxin reporter
DR5::GUS staining suggest that root system alteration and Pi-induced auxin response were at
least partially controlled by OsARF12.

These findings enrich our understanding of the biological functions of OsARF12, which also
acts in regulating Pi homeostasis.

Key words: auxin response factor, Oryza sativa (rice), OsARF12, phosphate absorption
and transport, phosphate homeostasis.

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Informasi Detail

Judul Seri

New Phytologist

No. Panggil

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Penerbit

: New Phytologist Trust, www.newphytologist.com., 2014

Deskripsi Fisik

New Phytologist (2014) 201: 91–103

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

Agus

Versi lain/terkait

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