e-journal

Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium

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The tomato receptor-like protein (RLP) Ve1 mediates resistance to
the vascular fungal pathogen Verticillium dahliae. To identify the
proteins required for Ve1 function, we transiently expressed
and immunopurified functional Ve1-enhanced green fluorescent
protein (eGFP) from Nicotiana benthamiana leaves, followed by
mass spectrometry. This resulted in the identification of peptides
originating from the endoplasmic reticulum (ER)-resident chaperones
HSP70 binding proteins (BiPs) and a lectin-type calreticulin
(CRT). Knock-down of the different BiPs and CRTs in tomato
resulted in compromised Ve1-mediated resistance to V. dahliae in
most cases, showing that these chaperones play an important
role in Ve1 functionality. Recently, it has been shown that one
particular CRT is required for the biogenesis of the RLP-type
Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing
of CRT3a resulted in a reduced pool of complex glycosylated
Cf-4 protein. In contrast, knock-down of the various CRTs in
N. benthamiana or N. tabacum did not result in reduced accumulation
of mature complex glycosylated Ve1 protein. Together, this
study shows that the BiP and CRT ER chaperones differentially
contribute to Cf-4- and Ve1-mediated immunity.

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Informasi Detail

Judul Seri

Molecular Plant Pathology

No. Panggil

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Penerbit

: BSPP AND JOHN WILEY & SONS LTD., 2014

Deskripsi Fisik

Molecular Plant Pathology, (2014) 15(1), 109–117

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

Agus

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