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Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato

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The brassinosteroid (BR) response transcription factor Brassinazole resistant 1 (BZR1)-mediated
BR signalling regulates many specific developmental processes including fruit ripening. Here, we
report the effect of 2,4-epibrassinolide (EBR) and BZR1-1D overexpression on carotenoid
accumulation and quality attributes of tomato (Solanum lycopersicum) fruit. EBR-treated pericarp
discs of ethylene-insensitive mutant, Never ripe, accumulated significantly more carotenoid
than those of the control. The results suggest that BR seems to be involved in modulating
pigments accumulation. When three independent transgenic lines overexpressing the Arabidopsis
BZR1-1D were used to evaluate the role of BZR1 in regulating tomato fruit carotenoid
accumulation and quality attributes, fruits of all three transgenic lines exhibited enhanced
carotenoid accumulation and increased soluble solid, soluble sugar and ascorbic acid contents
during fruit ripening. In addition, the fruits of two transgenic lines showed dark green shoulder
at mature green stage, in accordance with the up-regulated expression level of SlGLK2, which is
involved in chloroplast development. Our results indicate the importance of BZR1-centred BR
signalling in regulating carotenoid accumulation and quality attributes of tomato fruit and the
potential application of the BZR1-like(s) for improvement of nutritional quality and flavour of
tomato through genetic engineering.

Keywords: brassinosteroid, BZR1,carotenoid, fruit, quality attributes,Solanum lycopersicum (tomato).

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

: Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd., 2014

Deskripsi Fisik

Plant Biotechnology Journal (2014) 12, pp. 105–115

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

Agus

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