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Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

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The aim of this study was to evaluate the effect of low-level
laser therapy (LLLT) on odontoblast-like cells exposed to a
bleaching agent. Mouse dental papilla cell-23 cells were
seeded in wells of 24-well plates. Eight groups were established
according to the exposure to the bleaching agent and
LLLT (0, 4, 10 and 15 J cm
2). Enamel–dentin disks were
adapted to artificial pulp chambers, which were individually
placed in wells containing Dulbecco’s modified Eagle’s medium
(DMEM). A bleaching agent (35% hydrogen peroxide
[BA35%HP]) was applied on enamel (15 min) to obtain the
extracts (DMEM + BA35%HP components diffused through
enamel/dentin disks). The extracts were applied (1 h) to the
cells, and then subjected to LLLT. Cell viability (Methyl tetrazolium
assay), alkaline phosphatase (ALP) activity, as well
as gene expression of ALP, fibronectin (FN) and type I collagen,
were evaluated. The bleaching procedures reduced the
cell viability, ALP activity and gene expression of dentin proteins.
Laser irradiation did not modulate the cell response;
except for FN, as LLLT decreased the gene expression of this
protein by the cells exposed to the BA35%HP. It can be concluded
that BA35%HP decreased the activities of odontoblasts
that were not recovered by the irradiation of the
damaged cells with low-level laser parameters tested.

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Informasi Detail

Judul Seri

Photochemistry and Photobiology

No. Panggil

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Penerbit

: The American Society of Photobiology., 2014

Deskripsi Fisik

Photochemistry and Photobiology, 2014, 90: 201–206

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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