e-journal

Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing

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DNA methylation is essential for normal developmental processes and
genome stability. DNA methyltransferases are key enzymes catalyzing
DNA methylation. Chromomethylase (CMT) genes are specific to the
plant kingdom and encode chromodomain-containing methyltransferases.
However, the function of CMT genes in plants remains elusive. In this study,
we isolated and characterized a CMT gene from Nicotiana benthamiana,
designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with
other plant CMT3s showed conservation of bromo-adjacent-homology and
methyltransferase catalytic domains. We investigated the expression patterns
of NbCMT3 and its function in developmental programs. NbCMT3 was
expressed predominately in proliferating tissues such as apical shoots and
young leaves. NbCMT3 protein showed a nuclear location, which could be
related to its putative cellular functions. Knocking down NbCMT3 expression
by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis.
The formation of palisade cells was defective in NbCMT3-silenced plants as
compared with controls. NbCMT3 has a role in developmental programs.

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Informasi Detail

Judul Seri

Physiologia Plantarum

No. Panggil

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Penerbit

: Scandinavian Plant Physiology Society., 2014

Deskripsi Fisik

Physiologia Plantarum 150: 119–132. 2014

Bahasa

English

ISBN/ISSN

0031-9317

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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