e-journal

Glycine betaine-mediated potentiation of HSP gene expression involves calcium signaling pathways in tobacco exposed to NaCl stress

---

Glycine betaine (GB) can enhance heat tolerance and the accumulation of
heat-shock protein (HSP) in plants, but the effects of GB on HSP accumulation
during salt stress were not previously known. To investigate the mechanism
of how GB influences the expression of HSP, wild-type tobacco (Nicotiana
tabacum) seedlings pretreated with exogenous GB and BADH-transgenic
tobacco plants that accumulated GB in vivo were studied during NaCl
stress. A transient Ca2+ efflux was observed in the epidermal cells of
the elongation zone of tobacco roots after NaCl treatment for 1–2 min.
After 24 h of NaCl treatment, an influx of Ca2+ was observed; a low
concentration of GB significantly increased NaCl-induced Ca2+ influx. GB
increased the intracellular free calcium ion concentration and enhanced the
expression of the calmodulin (CaM) and heat-shock transcription factor (HSF)
genes resulting in potentiated levels of HSPs. Pharmacological experiments
confirmed that Ca2+ and CaM increased HSFs and HSPs gene expression,
which coincided with increased the levels of HSP70 accumulation. These
results suggest a mechanism by which GB acted as a cofactor in the NaCl
induction of a Ca2+-permeable current. A possible regulatory model of Ca2+-
CaM in the signal transduction pathway for induction of transcription and
translation of the active HSPs is described.

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Physiologia Plantarum

No. Panggil

-

Penerbit

: Scandinavian Plant Physiology Society., 2014

Deskripsi Fisik

Physiologia Plantarum 150: 63–75. 2014

Bahasa

English

ISBN/ISSN

0031-9317

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

PERTANIAN.

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar