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LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes

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The roles of a tomato (Lycopersicon esculentum) chloroplast‐targeted DnaJ protein (LeCDJ1) were investigated
using wild‐type (WT) and sense transgenic tomatoes. The LeCDJ1 expression was upregulated by 38 °C, 42 °C, 45 °C,NaCl, PEG, methyl viologen (MV) and hydrogen peroxide (H2O2), but not by 30 °C and 35 °C. Meanwhile, LeCDJ1 was involved in the response of plants to abscisic acid (ABA). Under heat stress, the sense plants showed better growth,higher chlorophyll content, lower malondialdehyde (MDA) accumulation and relative electrical conductivity (REC), and also less PSII photoinhibition than WT. Interestingly, the sense plants treated with streptomycin (SM), an inhibitor of organellar translation, still showed higher maximum photochemistry efficiency of PSII (Fv/Fm) and D1 protein levels than the SM‐untreated WT, suggesting that the protective effect of LeCDJ1 on PSII was, at least partially, independent of D1 protein synthesis. Furthermore, the relatively lower superoxide radical (O2 •
) and H2O2 levels in the sense plants were
considered to be due to the higher ascorbate peroxidase (APX) and superoxide dismutase (SOD) activity, which
seemed unlikely dependent on their transcription level. These results indicated that LeCDJ1 overexpression facilitated heat tolerance in transgenic tomatoes.

Keywords: D1 protein; DnaJ protein; heat stress; photosystem II;reactive oxygen species; tomato

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Informasi Detail

Judul Seri

J Integr Plant Biol

No. Panggil

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Penerbit

: Institute of Botany, Chinese Academy of Sciences., 2013

Deskripsi Fisik

J Integr Plant Biol 56: 63–74.

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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