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Molecular characterization of cisgenic lines of apple ‘Gala’ carrying the Rvi6 scab resistance gene

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Using resistance genes from a crossable donor to obtain cultivars resistant to diseases and the
use of such cultivars in production appears an economically and environmentally advantageous
approach. In apple, introgression of resistance genes by classical breeding results in new
cultivars, while introducing cisgenes by biotechnological methods maintains the original cultivar
characteristics. Recently, plants of the popular apple ‘Gala’ were genetically modified by inserting
the apple scab resistance gene Rvi6 (formerly HcrVf2) under control of its own regulatory
sequences. This gene is derived from the scab-resistant apple ‘Florina’ (originally from the wild
apple accession Malus floribunda 821). The vector used for genetic modification allowed a
postselection marker gene elimination to achieve cisgenesis. In this work, three cisgenic lines
were analysed to assess copy number, integration site, expression level and resistance to apple
scab. For two of these lines, a single insertion was observed and, despite a very low expression of
0.07- and 0.002-fold compared with the natural expression of ‘Florina’, this was sufficient to
induce plant reaction and reduce fungal growth by 80% compared with the scab-susceptible
‘Gala’. Similar results for resistance and expression analysis were obtained also for the third line,
although it was impossible to determine the copy number and TDNA integration site–such
molecular characterization is requested by the (EC) Regulation No. 1829/2003, but may become
unnecessary if cisgenic crops become exempt from GMO regulation.

Keywords: Venturia inaequalis, Malus 9 domestica, integration site, expression analysis, GMO.

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

: Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd., 2014

Deskripsi Fisik

Plant Biotechnology Journal (2014) 12, pp. 2–9

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

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