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Next-generation sequencing, FISH mapping and syntenybased modeling reveal mechanisms of decreasing dysploidy in Cucumis

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In the large Cucurbitaceae genus Cucumis, cucumber (C. sativus) is the only species with 2n = 2x = 14 chromosomes. The majority of the remaining species, including melon (C. melo) and the sister species of cucumber, C. hystrix, have 2n = 2x = 24 chromosomes, implying a reduction from n = 12 to n = 7. To understand
the underlying mechanisms, we investigated chromosome synteny among cucumber, C. hystrix and melon using integrated and complementary approaches. We identified 14 inversions and a C. hystrix lineage-specific
reciprocal inversion between C. hystrix and melon. The results reveal the location and orientation of 53
C. hystrix syntenic blocks on the seven cucumber chromosomes, and allow us to infer at least 59 chromosome
rearrangement events that led to the seven cucumber chromosomes, including five fusions, four
translocations, and 50 inversions. The 12 inferred chromosomes (AK1–AK12) of an ancestor similar to melon
and C. hystrix had strikingly different evolutionary fates, with cucumber chromosome C1 apparently resulting
from insertion of chromosome AK12 into the centromeric region of translocated AK2/AK8, cucumber chromosome C3 originating from a Robertsonian-like translocation between AK4 and AK6, and cucumber chromosome C5 originating from fusion of AK9 and AK10. Chromosomes C2, C4 and C6 were the result of complex reshuffling of syntenic blocks from three (AK3, AK5 and AK11), three (AK5, AK7 and AK8) and five (AK2, AK3, AK5, AK8 and AK11) ancestral chromosomes, respectively, through 33 fusion, translocation and inversion events. Previous results (Huang, S., Li, R., Zhang, Z. et al., 2009, Nat. Genet. 41, 1275–1281; Li, D.,Cuevas, H.E., Yang, L., Li, Y., Garcia-Mas, J., Zalapa, J., Staub, J.E., Luan, F., Reddy, U., He, X., Gong, Z., Weng, Y. 2011a, BMC Genomics, 12, 396) showing that cucumber C7 stayed largely intact during the entire evolution of Cucumis are supported. Results from this study allow a fine-scale understanding of the mechanisms of dysploid chromosome reduction that has not been achieved previously.


Keywords: chromosome evolution, comparative genome mapping, Cucumis, de novo genome sequence, dysploid chromosome number reduction, synteny.

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Informasi Detail

Judul Seri

The Plant Journal

No. Panggil

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Penerbit

: John Wiley & Sons, Ltd.., 2014

Deskripsi Fisik

The Plant Journal (2014) 77, 16–30

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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