e-journal

Pollen and seed transmission of Cucumber green mottle mosaic virus in cucumber

---

Cucumber green mottle mosaic virus (CGMMV) is transmitted mechanically in cucurbits, but whether it is transmitted via pollen to healthy plants and onto the subsequent generation of seedlings is unknown. Greenhouse experiments were conducted to investigate the importance of this route of infection. Cucumber seedlings at the 3-true-leaf stage were mechanically inoculated with CGMMV. At anthesis, scanning electron microscopy (SEM) and reverse transcription polymerase chain reaction (RT-PCR) were used to verify the presence of CGMMV. Pollen was collected from the flowers of the infected plants and used to fertilize non-inoculated plants. The rate of CGMMV transmission to the resulting fruits ranged from 17
1 to 51
2% compared with 33
3–100% for mechanically inoculated plants. Seeds were harvested from the cucumber fruits of both treatments and tested for the presence of CGMMV by RT-PCR. The CGMMV-positive seeds harvested from the two treatments were sown separately. The seed transmission rates for the inoculated and non-inoculated plants were 16
7–100% and 12
8–76
7%, respectively. It was concluded that CGMMV can be transmitted both horizontally via cucumber pollen and vertically, to the next generation, in infected seeds. In addition the rate of seed transmission was much higher than previous reports. These findings have important implications for the
disease management of CGMMV.

Keywords: Cucumber green mottle mosaic virus, pollen transmission, pollination, seed transmission, tobamovirus, transmission rate

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Plant Pathology

No. Panggil

-

Penerbit

London : British Society for Plant Pathology., 2014

Deskripsi Fisik

Plant Pathology (2014) 63, 72–77

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

PERTANIAN.

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar