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RhNAC3, a stress-associated NAC transcription factor, has a role in dehydration tolerance through regulating osmotic stress-related genes in rose petals

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Petal cell expansion depends on cell wall metabolism, changes in cell turgor pressure and
restructuring of the cytoskeleton, and recovery ability of petal cell expansion is defined as
an indicator of dehydration tolerance in flowers. We previously reported that RhNAC2, a
development-related NAC domain transcription factor, confers dehydration tolerance through
regulating cell wall-related genes in rose petals. Here, we identify RhNAC3, a novel rose SNAC
gene, and its expression in petals induced by dehydration, wounding, exogenous ethylene and
abscisic acid (ABA). Expression studies in Arabidopsis protoplasts and yeast show that RhNAC3
has transactivation activity along its full length and in the carboxyl-terminal domain. Silencing
RhNAC3 in rose petals by virus-induced gene silencing (VIGS) significantly decreased the cell
expansion of rose petals under rehydration conditions. In total, 24 of 27 osmotic stress-related
genes were down-regulated in RhNAC3-silenced rose petals, while only 4 of 22 cell
expansion-related genes were down-regulated. Overexpression of RhNAC3 in Arabidopsis
gave improved drought tolerance, with lower water loss of leaves in transgenic plants.
Arabidopsis ATH1 microarray analysis showed that RhNAC3 regulated the expression of stressresponsive
genes in overexpressing lines, and further analysis revealed that most of the
RhNAC3-up-regulated genes were involved in the response to osmotic stress. Comparative
analysis revealed that different transcription regulation existed between RhNAC3 and
RhNAC2. Taken together, these data indicate that RhNAC3, as a positive regulator, confers
dehydration tolerance of rose petals mainly through regulating osmotic adjustment-associated
genes.

Keywords: cell expansion, dehydration tolerance, NAC transcription factor, osmotic adjustment, transcriptional regulation,rose flower.

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

: Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd., 2014

Deskripsi Fisik

Plant Biotechnology Journal (2014) 12, pp. 38–48

Bahasa

English

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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