Study on a biomimetic enzyme-linked immunosorbent assay method for rapid determination of trace acrylamide in French fries and cracker samples

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Study on a biomimetic enzyme-linked immunosorbent assay method for rapid determination of trace acrylamide in French fries and cracker samples

Qing Sun - Nama Orang; Longhua Xu - Nama Orang; Yue Ma - Nama Orang; Xuguang Qiao - Nama Orang; Zhixiang Xu - Nama Orang;

BACKGROUND: Acrylamide has attracted worldwide concern due to its neurotoxicity, genotoxicity, reproductive–development toxicity. It is necessary to develop an accurate and reliable analytical method to prevent the harm on the human health.
RESULTS: In this study, a sensitive and fast analytical method of direct competitive biomimetic enzyme-linked immunosorbent assay(BELISA)wasdevelopedusingahydrophilicimprintedmembraneasbiomimeticantibody. Thisnovelimprintedmembrane was directly synthesised on the well surface of MaxiSorp polystyrene 96-well plates in an aqueous environment, and it exhibited high binding ability and specificity toward acrylamide. Under the optimal conditions, the established BELISA method had a good sensitivity (IC50, 8.0±0.4 mg L−1) and a low limit of detection (IC15, 85.0±4.2 μg L−1). The blank potato samples spiked with acrylamide at three levels of 100, 250 and 500 μg L−1 were extracted and determinate by the proposed method, and good recoveries ranging from 90.0% to 110.5% were obtained. This presented method was applied to the quantitative detection
of the acrylamide in French fries and cracker samples. Also, the results were correlated well with that obtained by the gas chromatography method.
CONCLUSION: With good properties of high sensitivity, simple pre-treatment and low cost, this BELISA could be a promising screening method in food sample analysis.

Keywords: hydrophilic imprinted membrane; biomimetic antibody; enzyme-linked immunosorbent assay; acrylamide

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Judul Seri: J Sci Food Agric
No. Panggil: -
Penerbit: : Society of Chemical Industry., 2014
Deskripsi Fisik: J Sci Food Agric 2014; 94: 102–108
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: PERTANIAN.
Info Detail Spesifik: -
Pernyataan Tanggungjawab: agus

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Study on a biomimetic enzyme-linked immunosorbent assay method for rapid determination of trace acrylamide in French fries and cracker samples

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