e-journal

Mutation of Arabidopsis CATALASE2 results in hyponastic leaves by changes of auxin levels

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ABSTRACT
Auxin and H2O2 play vital roles in plant development and environmental responses; however, it is unclear whether and how H2O2 modulates auxin levels. Here, we investigate this question using cat2-1 mutant, which exhibits reduced catalase activity and accumulates high levels ofH2O2 under photorespiratory conditions. At a light intensity of 150 mmol m-2 s-1, the mutant exhibited up-curled leaves that have increased H2O2 contents and decreased auxin levels. At low light intensities (30 mmol m-2 s-1), the leaves of the mutant were normal, but exhibited reduced H2O2 contents and elevated auxin levels. These findings suggest thatH2O2 modulates auxin levels.When auxin was directly applied to cat2-1 leaves, the up-curled leaves curled downwards. In addition, transformation of cat2-1 plants with pCAT2:iaaM, which increases auxin levels, rescued the
hyponastic leaf phenotype. Using qRT-PCR,we demonstrated that the transcription of auxin synthesis-related genes and of genes that regulate leaf curvature is suppressed in cat2-1. Furthermore, application of glutathione rescued the up-curled leaves of cat2-1 and increased auxin levels, but did not change H2O2 levels. Thus, the hyponastic leaves of cat2-1 reveal crosstalk between H2O2 and auxin signalling that is mediated by changes in glutathione redox status.

Key-words: cat2; glutathione redox status.

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Ketersediaan

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Informasi Detail

Judul Seri

Plant, Cell and Environment

No. Panggil

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Penerbit

: JohnWiley & Sons Ltd., 2014

Deskripsi Fisik

Plant, Cell and Environment (2014) 37, 175–188

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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