e-journal
Self-Powered Enzyme-Based Biosensors
Electrical contacting of redox enzymes with electrode supports attracts substantial research efforts directed to the development of biosensors, electrobiocatalyzed chemical transformations,and the development of biofuel cell elements. Tethering of electroactive relays to redox proteins or the immobilization of redox proteins in electroactive polymers6 are common practices to electrically contact and activate the redox enzymes. Recently, we reported on the effective electrical contacting of flavoenzymes on electrodes by their structural alignment on electrodes through the surface reconstitution of apoflavoenzymes on a relay-FAD
monolayer assembly. This concept was further generalized by tailoring integrated, electrically contacted, cofactor-dependent enzyme electrodes by the cross-linking of affinity complexes between NAD+-dependent enzymes and an electrocatalyst-NAD+monolayer associated with electrodes.
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