e-journal

Agrobacterium-Mediated Transformation of Switchgrass and Inheritance of the Transgenes

---

Abstract
Switchgrass (Panicum virgatum L.) has been developed into an important biofuel crop. Embryogenic calli
induced from caryopses or inflorescences of the lowland switchgrass cultivar Alamo were used for Agrobacteriummediated transformation. A chimeric hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin as the selection agent. Embryogenic calli were infected with Agrobacterium tumefaciens strain EHA105. Calli resistant to hygromycin were obtained after 5 to 8 weeks of selection. Soil-grown transgenic switchgrass plants were obtained 4 to 5 months after Agrobacterium infection. The transgenic nature of the regenerated plants was demonstrated by PCR, Southern blot hybridization analysis, and GUS staining. T1 progeny were obtained after reciprocal crosses between transgenic and untransformed control plants. Molecular analyses of the T1 progeny revealed various patterns of segregation. Transgene silencing was observed in the progeny with multiple inserts. Interestingly, reversal of the expression of the silenced transgene was found in segregating progeny with a single insert.

Keywords: Agrobacterium . Biofuel crop . Panicum virgatum . Switchgrass . Transgene inheritance . Transgenic plant

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Bioenerg. Res.

No. Panggil

-

Penerbit

New York : Springer Science Business Media., 2009

Deskripsi Fisik

Bioenerg. Res. (2009) 2:275–283

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar