e-journal
Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production
Abstract.
Bermudagrass, reed and rapeseed were pretreated with phosphoric acid–acetone and used for ethanol
production by means of simultaneous saccharification and fermentation (SSF) with a batch and fed-batch
mode. When the batch SSF experiments were conducted in a 3% low effective cellulose, about 16 g/L of
ethanol were obtained after 96 h of fermentation. When batch SSF experiments were conducted with a
higher cellulose content (10% effective cellulose for reed and bermudagrass and 5% for rapeseed), higher
ethanol concentrations and yields (of more than 93%) were obtained. The fed-batch SSF strategy was
adopted to increase the ethanol concentration further. When a higher water-insoluble solid (up to
36%) was applied, the ethanol concentration reached 56 g/L of an inhibitory concentration of the yeast
strain used in this study at 38 oC. The results show that the pretreated materials can be used as good feedstocks for bioethanol production, and that the phosphoric acid–acetone pretreatment can effectively
yield a higher ethanol concentration.
Keywords: Simultaneous saccharification, fermentation, Lignocellulose, Pretreatment, Phosphoric acid–acetone,
Bioethanol
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