e-journal

Characterization and micellization of rhamnolipidic fractions and crude extracts produced by Pseudomonas aeruginosa mutant MIG-N146

---

Abstract.
Two representative rhamnolipidic fractions, RL-F1 and RL-F2, produced by the P. aeruginosa mutant strain
MIG-N146, were separated and chemically characterized by TLC, HPLC-MS, and FTIR. The RL-F1 fraction is
predominantly mono-rhamnolipid homologues with a high content of one or two fatty acid moieties. The
RL-F2 fraction is mainly composed of di-rhamnosyl moieties with two hydrophobic tails. Micellization
behavior was investigated to assess the physicochemical properties of the surfactants, RL-F1, RL-F2,
and crude rhamnolipidic extracts. The variations in morphology of micelle formation and growth were
examined by dynamic light scattering measurements as a function of surfactant concentration. Critical
micelle concentration (CMC), average minimal surface tension (γCMC), saturated surface excess (Γm), mean
surface area per molecule (S), and adsorption efficiency (pC20) were determined from the surface tension
profiles and compared for the three surfactant systems. It was found that micelle growth was significantly
enhanced by increasing rhamnolipid bulk concentration, which was most probably accompanied with
an aggregate shape transition. Well-separated multi- or bi-modal distributions of particle size were
observed in RL-F2 and the crude extracts solutions. The results of this study demonstrate that molecular
architecture of different surfactant compositions profoundly influences the performance of rhamnolipidic
surfactants.

Keywords:
Rhamnolipids
HPLC-MS
Homologues
Surface property
Micellization behavior

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Journal of Colloid and Interface Science

No. Panggil

-

Penerbit

London : Elsevier Inc.., 2009

Deskripsi Fisik

Journal of Colloid and Interface Science 331 (2009) 356–363

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar