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Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1

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Abstract.
A bacterial strain C2A1 isolated from soil was found highly effective in degrading chlorpyrifos and its
first hydrolysis metabolite 3,5,6-trichloro-2-pyridinol (TCP). On the basis of morphology, physiological
characteristics, biochemical tests and 16S rRNA sequence analysis, strain C2A1 was identified as Bacillus
pumilus. Role of strain C2A1 in the degradation of chlorpyrifos was examined under different culture
conditions like pH, inoculum density, presence of added carbon/nutrient sources and pesticide concentration.
Chlorpyrifos was utilized by strain C2A1 as the sole source of carbon and energy as well as it was
co-metabolized in the presence of glucose, yeast extract and nutrient broth. Maximum pesticide degradation
was observed at high pH (8.5) and high inoculum density when chlorpyrifos was used as the sole
source and energy. In the presence of other nutrients, chlorpyrifos degradation was enhanced probably
due to high growth on easily metabolizable compounds which in turn increased degradation. The strain
C2A1 showed 90% degradation of TCP (300 mgL−1) within 8 days of incubation.

Keywords:
Pesticides
Organophosphate
Chlorpyrifos
Biodegradation
3,5,6-Trichloro-2-pyridinol (TCP)
Bacillus pumilus

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Informasi Detail

Judul Seri

Journal of Hazardous Materials

No. Panggil

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Penerbit

London : Elsevier B.V.., 2009

Deskripsi Fisik

Journal of Hazardous Materials 168 (2009) 400–405

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

LINGKUNGAN HIDUP

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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