e-journal

Influence of carbon nanotube scaffolds on human cervical carcinoma HeLa cell viability and focal adhesion kinase expression

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Abstract.
Five types of carbon nanotube (CNT) scaffolds were prepared by vacuum filtration of the
dispersed single-wall carbon nanotubes (SWCNTs), acid-treated SWCNTs, multi-wall carbon
nanotubes (MWCNTs), acid-treated MWCNTs, and amylose-wrapped SWCNTs in water
onto porous poly(vinylidene chloride) membranes. The influence of these scaffolds on
human cervical carcinoma HeLa cells was investigated by WST-1 assay, acridine orange/
ethidium bromide double staining and 1,10-dioctadecyl-3,3,30,30tetram-ethylindocarbocyanine
perchlorate staining. The results indicated that the viability of HeLa cells cultured
on these scaffolds decreased in the following order: amylose-wrapped SWCNTs > acid-treated
MWCNTs > MWCNTs > acid-treated SWCNTs > SWCNTs. Cells cultured on SWCNTs
and on acid-treated SWCNTs were found undergoing apoptosis with damaged cell membrane
and condensed chromatin. The result of an immunocytochemical test showed that
both ‘‘dot-like’’ and ‘‘dash-like’’ focal adhesion kinases (FAKs) mainly distributed at the
periphery of cells cultured on SWCNTs, while ‘‘dot-like’’ FAKs distributed in the whole cell
body of cells cultured on MWCNTs. We therefore hypothesize that FAK expression might
play a key role in controlling cell viability for cells cultured on CNT scaffolds.

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Informasi Detail

Judul Seri

C A R B ON

No. Panggil

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Penerbit

London : Elsevier Ltd.., 2008

Deskripsi Fisik

C A R B ON 4 6 ( 2 0 0 8 ) 4 5 3 –4 6 0

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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