e-journal

Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles

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Abstract.
To explore the effects of the novel properties of carbon nanoparticles (CNPs) on cytotoxicity,
the adsorption of serum proteins in cell culture medium on multi-walled carbon nanotubes
and three kinds of carbon blacks was investigated. The uptake of CNPs by Hela cells
was measured quantitatively using 99mTc radionuclide labeling and tracing techniques, and
the dependence of CNPs uptake on serum proteins was examined. The cytotoxicity of CNPs
in medium with and without serum was assayed by the MTT [3-(4,5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] method. It was found that cellular uptake was much
higher in cells exposed to CNPs in serum-free culture medium than those in culture medium
with serum. Serum proteins adsorbed on CNPs attenuated the inherent cytotoxicity of
CNPs, and the extent of toxicity attenuation increased with increasing amounts of serum
proteins adsorbed on CNPs. The possible reasons responsible for the considerable influence
of serum proteins on cytotoxicity were indicated.

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Informasi Detail

Judul Seri

C A R B ON

No. Panggil

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Penerbit

London : Elsevier Ltd.., 2009

Deskripsi Fisik

C A R B ON 4 7 ( 2 0 0 9 ) 1 3 5 1 –1 3 5 8

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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