e-journal

Class III peroxidases are activated in proanthocyanidin-deficient Arabidopsis thaliana seeds

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Background and Aims:
It has previously been shown that proanthocyanidins (PAs) in the seed coat of Arabidopsis thaliana have the ability to scavenge superoxide radicals (O2-). However, the physiological processess in PA-deficit seeds are not clear. It is hypothesized that there exist alternative ways in PA-deficient seeds to cope with oxidative stress.
Methods:
The content of hydrogen peroxide (H2O2) and its relevance to the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidases was investigated in both wild-type and PA-deficit mutant seeds. A biochemical
staining approach was used to detect tissue localizations of peroxidase activities in PA-deficit mutant
seeds.
Key Results:
PA-deficient mutants possess significantly lower levels of H2O2 than the wild-type, despite their higher accumulation of superoxide radicals. Screening of the key antioxidant enzymes revealed that peroxidase
activity was significantly over-activated in mutant seeds. This high peroxidase activity was mainly confined to the seed coat zone. Interestingly, neither ascorbate peroxidase nor glutathione peroxidase, just the guaiacol peroxidases (class III peroxidases), was specifically activated in the seed coat. However, no significant difference in peroxidase activity was observed in embryos of either mutants or the wild-type, although gene expressions of several candidate peroxidases were down-regulated in the embryos of PA-deficient seeds.
Conclusions:
The results suggest that enhanced class III peroxidase activity in the seed coat of PA-deficient
mutants is an adaptive strategy for seed development and survival.

Key words: Proanthocyanidins, peroxidase, seed coat, seed development, seed germination, anti- oxidation,
Arabidopsis thaliana.

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Ketersediaan

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2013

Deskripsi Fisik

Annals of Botany 111: 839–847, 2013

Bahasa

English

ISBN/ISSN

10958290

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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