e-journal

Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment

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Abstract.
As interest in howto increase biomass production through biotechnologicalmeans gains traction, focus is
turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevatedCO2 with cold cathode fluorescent lamps (CCFLs)on the photoautotrophic growth of Oncidesa in vitro has been studied using a gas-permeable film culture vessel, the ‘Vitron’. The growth of Oncidesa (formerly Oncidesa Gower Ramsey ‘U-1’) plantlets on Vacin andWent (VW)medium was stimulated by 10 000 μmol mol-1 CO2. In particular, increasing the photosynthetic photon flux density (PPFD) from 45 to 60 μmol m-2 s-1 under 10 000 μmol mol-1 CO2 in the growth chamber remarkably increased the number of leaves and roots, and shoot and root fresh and dry weights compared with plantlets under the same level of CO2 under low PPFD (45 μmol m-2 s-1). However, there was a remarkable decrease in photosynthetic capacity, and chlorosis and browning of leaves. In stark contrast, plantlets grown on Kyoto medium at 10 000 μmol mol-1 CO2 under high PPFD had a higher photosynthetic rate than plantlets grown on VW medium, and no chlorosis or browningwas observed. Furthermore, shoot growth was remarkably enhanced. Therefore, super-elevated CO2 (10 000 μmol mol-1) enrichment and growth under CCFLs can positively affect the efficiency and quality of commercial production of clonal Oncidesa plantlets.

Keywords: CCFL; Oncidesa (formerly Oncidesa Gower Ramsey ‘U-1’); photoautotrophic growth; single-leaf photosynthesis; super-elevated CO2.

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Informasi Detail

Judul Seri

AoB PLANTS

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2013

Deskripsi Fisik

AoB PLANTS 5: plt044; doi:10.1093/aobpla/plt044

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

5: plt044

Subjek

KULTUR JARINGAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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