e-journal

Identification of Stylosanthes guianensis varieties using molecular genetic analysis

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Abstract.
Background and aims:
The botanical classification of Stylosanthes guianensis is controversial, and few studies have
used molecular markers to analyse this species. We used microsatellite markers to study
the genetic diversity and population structure of S. guianensis and compare our results
with the current infraspecific botanical classification.

Methodology:
A representative sample from the S. guianensis Brazilian germplasm collection (150 accessions)
was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented
in the software STRUCTURE was used to assign accessions into clusters. A
dendrogram was constructed based on Roger’s genetic distances.

Principal results:
The number of alleles per locus varied from 2 to 11, with an average of 4.7. The observed (HO)
and expected (HE) heterozygosity values varied from 0 to 0.58 (mean of 0.18) and from 0.04 to
0.83 (mean of 0.55), respectively. Nine groups were assembled in STRUCTURE, and these
groups were consistent with clusters inferred from the genetic distances and taxonomic
varieties described for S. guianensis. The GST among the nine groups was 0.46.

Conclusions:
The low HO and the GST values observed are in agreement with the outcrossing rate (26 %)
estimated for this species. The data indicate a high genetic diversity among and within the
botanical varieties and suggest that microsatellite-based information can be combined
with classical taxonomy to elucidate infraspecific levels.

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Informasi Detail

Judul Seri

AoB PLANTS

No. Panggil

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Penerbit

Oxford : Oxford University Press., 2012

Deskripsi Fisik

AoB PLANTS 2012: pls001; doi:10.1093/aobpla/pls001

Bahasa

English

ISBN/ISSN

-

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2012: pls001

Subjek

PEMULIAAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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