e-journal

Micropropagation of Ilex khasiana, a critically endangered and endemic holly of Northeast India

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Abstract.
Background and aims:
Ilex khasiana is a rare and critically endangered holly endemic to the Khasi Hills of Meghalaya,
India, and confined to a small number of pocket areas. In addition to conventional methods
of propagation, endemic and threatened plants such as this could be more effectively multiplied
and conserved using in vitro methods. Such techniques have the additional advantage of
having a low impact on wild populations because they require a minimum of starting
material. Our objective was to develop methodologies for the successful in vitro mass propagation
of I. khasiana.

Methodology:
Seedlings were germinated in vitro under sterile conditions and nodal explants from these
were transferred to Murashige and Skoog (MS) medium supplemented with 8.88 μM 6-benzyladenine
and 4.64 μM kinetin.

Principal results:
This generated 10 shoots per explant. In a second approach, callus was obtained from seedling-
derived leaf discs cultured on MS medium supplemented with 2,4-dichlorophenoxyacetic
acid and 6-benzyladenine. Approximately 12 adventitious shoots per callus were regenerated
from 83.33 % of the calli after transfer to MS medium supplemented with 6.63 μM 6-benzyladenine.
The most effective treatment for inducing root formation on the shoots was transfer
of shoots to half-strength MS medium with 9.84 μM indole-3-butyric acid. Regenerated plantlets
with well-developed shoots and roots were hardened and transferred to open soil with
70 % survival after 4 weeks.

Conclusions:
Both the methods described here are well suited for the mass multiplication of this critically
endangered tree species.

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Informasi Detail

Judul Seri

AoB PLANTS

No. Panggil

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Penerbit

Oxford : Oxford University Press., 2011

Deskripsi Fisik

AoB PLANTS 2011 plr012 doi:10.1093/aobpla/plr012

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2011 plr012

Subjek

PEMULIAAN TANAMAN
PERSEBARAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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