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Microsatellite markers for an endemic Atlantic Forest tree, Manilkara multifida (Sapotaceae)

Abstract.
Manilkara multifida is a tropical tree that is endemic to the Atlantic forests of southern Bahia, Brazil. Currently, populations of this species are restricted to fragmented landscapes that are susceptible to anthropogenic disturbances. Considering this issue, and that there is no genetic information available for this endangered species, we developed microsatellite markers for M. multifida to provide resources for future conservation genetics studies. Using an enriched genomic library, we isolated eight polymorphic microsatellite loci and optimized the amplification conditions for M. multifida. For each locus, we estimated the number of alleles, HE and HO, paternity exclusion Q, individual identity I and fixation index F, and examined the presence of null alleles. The mean number of alleles was 11.9, and the heterozygosity was high at all loci (average HE = 0.809 and HO = 0.777). The combined values for both paternity exclusion and individual identity were Q = 0.9959 and I = 5.45 × 10–11, respectively. No evidence of null alleles was detected. The results of our analysis indicated that all eight microsatellites are promising for assessing questions involving inbreeding, gene flow, co-ancestry and mating patterns in M. multifida.

Keywords: Conservation; fragmentation; molecular ecology; molecular marker; PCR; population genetics; SSR;
tropical rainforest.

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Informasi Detail
Judul Seri
AoB PLANTS
No. Panggil
-
Penerbit
Oxford : Oxford University Press on behalf of the Annals of Botany Company.,
Deskripsi Fisik
AoB PLANTS 5: plt006; doi:10.1093/aobpla/plt006
Bahasa
English
ISBN/ISSN
-
Klasifikasi
-
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
5: plt006
Subjek
PEMULIAAN TANAMAN
EKOLOGI TANAMAN
Info Detail Spesifik
-
Pernyataan Tanggungjawab
Versi lain/terkait

Tidak tersedia versi lain

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