e-journal

Molecular detection and species identification of Alexandrium (Dinophyceae) causing harmful algal blooms along the Chilean coastline

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Abstract.
Background and aims:
On the basis of morphological evidence, the species involved in South American Pacific coast
harmful algal blooms (HABs) has been traditionally recognized as Alexandrium catenella
(Dinophyceae). However, these observations have not been confirmed using evidence
based on genomic sequence variability. Our principal objective was to accurately determine
the species of Alexandrium involved in local HABs in order to implement a real-time polymerase
chain reaction (PCR) assay for its rapid and easy detection on filter-feeding shellfish, such
as mussels.

Methodology:
For species-specific determination, the intergenic spacer 1 (ITS1), 5.8S subunit, ITS2 and the
hypervariable genomic regions D1–D5 of the large ribosomal subunit of local strains were
sequenced and compared with two data sets of other Alexandrium sequences. Speciesspecific
primers were used to amplify signature sequences within the genomic DNA of the
studied species by conventional and real-time PCR.

Principal results:
Phylogenetic analysis determined that the Chilean strain falls into Group I of the tamarensis
complex. Our results support the allocation of the Chilean Alexandrium species as a toxic Alexandrium
tamarense rather than A. catenella, as currently defined. Once local species were
determined to belong to Group I of the tamarensis complex, a highly sensitive and accurate
real-time PCR procedure was developed to detect dinoflagellate presence in Mytilus spp.
(Bivalvia) samples after being fed (challenged) in vitro with the Chilean Alexandrium strain.
The results show that real-time PCR is useful to detect Alexandrium intake in filter-feeding
molluscs.

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Informasi Detail

Judul Seri

AoB PLANTS

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2012

Deskripsi Fisik

AoB PLANTS 2012: pls033; doi:10.1093/aobpla/pls033

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2012: pls033

Subjek

PEMULIAAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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