e-journal

Parentage determination of Vanda Miss Joaquim (Orchidaceae) through two chloroplast genes rbcL and matK

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Abstract.
Background and aims:
The popular hybrid orchid Vanda Miss Joaquim was made Singapore’s national flower in 1981.
It was originally described in the Gardeners’ Chronicle in 1893, as a cross between Vanda
hookeriana and Vanda teres. However, no record had been kept as to which parent contributed
the pollen. This study was conducted using DNA barcoding techniques to determine the
pod parent of V. Miss Joaquim, thereby inferring the pollen parent of the hybrid by exclusion.
Methodology Two chloroplast genes, matK and rbcL, from five related taxa, V. hookeriana, V. teres var. alba,
V. teres var. andersonii, V. teres var. aurorea and V. Miss Joaquim ‘Agnes’, were sequenced. The
matK gene from herbarium specimens of V. teres and V. Miss Joaquim, both collected in 1893,
was also sequenced.

Principal results:
No sequence variation was found in the 600-bp region of rbcL sequenced. Sequence variation
was found in the matK gene of V. hookeriana, V. teres var. alba, V. teres var. aurorea and
V. Miss Joaquim ‘Agnes’. Complete sequence identity was established between V. teres var.
andersonii and V. Miss Joaquim ‘Agnes’. The matK sequences obtained from the herbarium
specimens of V. teres and V. Miss Joaquim were completely identical to the sequences
obtained from the fresh samples of V. teres var. andersonii and V. Miss Joaquim ‘Agnes’.

Conclusions:
The pod parent of V. Miss Joaquim ‘Agnes’ is V. teres var. andersonii and, by exclusion, the
pollen parent is V. hookeriana. The herbarium and fresh samples of V. teres var. andersonii
and V. Miss Joaquim share the same inferred maternity. The matK gene was more informative
than rbcL and facilitated differentiation of varieties of V. teres.

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Informasi Detail

Judul Seri

AoB PLANTS

No. Panggil

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Penerbit

Oxford : Oxford University Press., 2011

Deskripsi Fisik

AoB PLANTS 2011 plr018 doi:10.1093/aobpla/plr018

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2011 plr018

Subjek

PEMULIAAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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