e-journal

Differential expression of microRNAs and other small RNAs in barley between water and drought conditions

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Summary.
Drought is a major constraint to crop production, and microRNAs (miRNAs) play an important
role in plant drought tolerance. Analysis of miRNAs and other classes of small RNAs (sRNAs)
in barley grown under water and drought conditions reveals that drought selectively regulates
expression of miRNAs and other classes of sRNAs. Low-expressed miRNAs and all repeatassociated
siRNAs (rasiRNAs) tended towards down-regulation, while tRNA-derived sRNAs
(tsRNAs) had the tendency to be up-regulated, under drought. Antisense sRNAs (putative
siRNAs) did not have such a tendency under drought. In drought-tolerant transgenic barley
overexpressing DREB transcription factor, most of the low-expressed miRNAs were also downregulated.
In contrast, tsRNAs, rasiRNAs and other classes of sRNAs were not consistently
expressed between the drought-treated and transgenic plants. The differential expression
of miRNAs and siRNAs was further confirmed by Northern hybridization and quantitative
real-time PCR (qRT-PCR). Targets of the drought-regulated miRNAs and siRNAs were
predicted, identified by degradome libraries and confirmed by qRT-PCR. Their functions
are diverse, but most are involved in transcriptional regulation. Our data provide insight into
the expression profiles of miRNAs and other sRNAs, and their relationship under drought,
thereby helping understand how miRNAs and sRNAs respond to drought stress in cereal crops.

Keywords: barley, drought, microRNA, small RNA, differential expression.

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Ketersediaan

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

London : Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd., 2015

Deskripsi Fisik

Plant Biotechnology Journal (2015) 13, pp. 2–13

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOTEKNOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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