e-journal

Overexpression of a rice heme activator protein gene (OsHAP2E) confers resistance to pathogens, salinity and drought, and increases photosynthesis and tiller number

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Summary.
Heme activator protein (HAP), also known as nuclear factor Y or CCAAT binding factor (HAP/NFY/
CBF), has important functions in regulating plant growth, development and stress responses.
The expression of rice HAP gene (OsHAP2E) was induced by probenazole (PBZ), a chemical
inducer of disease resistance. To characterize the gene, the chimeric gene (OsHAP2E::GUS)
engineered to carry the structural gene encoding b-glucuronidase (GUS) driven by the promoter
from OsHAP2E was introduced into rice. The transgenic lines of OsHAP2Ein::GUS with the intron
showed high GUS activity in the wounds and surrounding tissues. When treated by salicylic acid
(SA), isonicotinic acid (INA), abscisic acid (ABA) and hydrogen peroxide (H2O2), the lines showed
GUS activity exclusively in vascular tissues and mesophyll cells. This activity was enhanced after
inoculation with Magnaporthe oryzae or Xanthomonas oryzae pv. oryzae. The OsHAP2E
expression level was also induced after inoculation of rice with M. oryzae and X. oryzae pv.
oryzae and after treatment with SA, INA, ABA and H2O2, respectively. We further produced
transgenic rice overexpressing OsHAP2E. These lines conferred resistance to M. oryzae or X.
oryzae pv. oryzae and to salinity and drought. Furthermore, they showed a higher photosynthetic
rate and an increased number of tillers. Microarray analysis showed up-regulation of
defence-related genes. These results suggest that this gene could contribute to conferring biotic
and abiotic resistances and increasing photosynthesis and tiller numbers.

Keywords: heme activator protein 2 (HAP2), rice, biotic stress, abiotic stress, photosynthesis, CCAAT.

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

: Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd., 2015

Deskripsi Fisik

Plant Biotechnology Journal (2015) 13, pp. 85–96

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOTEKNOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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