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Pathogen-induced SGT1 of Arachis diogoi induces cell death and enhanced disease resistance in tobacco and peanut

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Summary.
We have identified a transcript derived fragment (TDF) corresponding to SGT1 in a study of
differential gene expression on the resistant wild peanut, Arachis diogoi, upon challenge from
the late leaf spot pathogen, Phaeoisariopsis personata, and cloned its full-length cDNA followed
by subsequent validation through q-PCR. Sodium nitroprusside, salicylic acid, ethephon and
methyl jasmonate induced the expression of AdSGT1, while the treatment with abscisic acid did
not elicit its up-regulation. AdSGT1 is localized to both nucleus and cytoplasm. Its overexpression
induced hypersensitive-like cell death in tobacco under transient conditional expression using the
estradiol system, and this conditional expression of AdSGT1 was also associated with the upregulation
of NtHSR203J, HMGR and HIN1, which have been shown to be associated with
hypersensitive response in tobacco in earlier studies. Expression of the cDNA in a susceptible
cultivated peanut variety enhanced its resistance against the late leaf spot pathogen,
Phaeoisariopsis personata, while the heterologous expression in tobacco enhanced its resistance
against Phytophthora parasitica var. nicotianae, Alternaria alternata var. nicotianae and
Rhizoctonia solani. Constitutive expression in peanut was associated with the co-expression of
resistance-related genes, CC-NB-LRR and some protein kinases.

Keywords: AdSGT1, transgenic peanut, late leaf spot, tobacco, hypersensitive-like cell death.

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

London : Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd., 2015

Deskripsi Fisik

Plant Biotechnology Journal (2015) 13, pp. 73–84

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOTEKNOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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