e-journal

Transcriptome sequencing of different narrow-leafed lupin tissue types provides a comprehensive uni-gene assembly and extensive gene-based molecular markers

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Summary.
Narrow-leafed lupin (NLL; Lupinus angustifolius L.) is an important grain legume crop that is
valuable for sustainable farming and is becoming recognized as a human health food. NLL
breeding is directed at improving grain production, disease resistance, drought tolerance and
health benefits. However, genetic and genomic studies have been hindered by a lack of
extensive genomic resources for the species. Here, the generation, de novo assembly and
annotation of transcriptome datasets derived from five different NLL tissue types of the
reference accession cv. Tanjil are described. The Tanjil transcriptome was compared to
transcriptomes of an early domesticated cv. Unicrop, a wild accession P27255, as well as
accession 83A:476, together being the founding parents of two recombinant inbred line (RIL)
populations. In silico predictions for transcriptome-derived gene-based length and SNP
polymorphic markers were conducted and corroborated using a survey assembly sequence for
NLL cv. Tanjil. This yielded extensive indel and SNP polymorphic markers for the two RIL
populations. A total of 335 transcriptome-derived markers and 66 BAC-end sequence-derived
markers were evaluated, and 275 polymorphic markers were selected to genotype the
reference NLL 83A:476 9 P27255 RIL population. This significantly improved the
completeness, marker density and quality of the reference NLL genetic map.

Keywords: lupin, indel, SNP, molecular marker, next-generation sequencing.

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Informasi Detail

Judul Seri

Plant Biotechnology Journal

No. Panggil

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Penerbit

London : Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd., 2015

Deskripsi Fisik

Plant Biotechnology Journal (2015) 13, pp. 14–25

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOTEKNOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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