e-journal

Pectic-β(1,4)-galactan, extensin and arabinogalactan–protein epitopes differentiate ripening stages in wine and table grape cell walls

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† Background and Aims:
Cell wall changes in ripening grapes (Vitis vinifera) have been shown to involve re-modelling of pectin, xyloglucan and cellulose networks. Newer experimental techniques, such as molecular probes specific for cellwall epitopes, haveyet to be extensively used in grape studies. Limited general information is available on the cell wall properties that contribute to texture differences between wine and table grapes. This study evaluates whether profiling tools can detect cell wall changes in ripening grapes from commercial vineyards.

† Methods:
Standard sugar analysis and infra-red spectroscopy were used to examine the ripening stages (green, veraison and ripe) in grapes collected from Cabernet Sauvignon and Crimson Seedless vineyards. Comprehensive microarray polymer profiling (CoMPP) analysis was performed on cyclohexanediaminetetraacetic acid (CDTA)
and NaOH extracts of alcohol-insoluble residue sourced from each stage using sets of cell wall probes (mAbs and CBMs), and the datasets were analysed using multivariate software.

† Key Results:
The datasets obtained confirmed previous studies on cell wall changes known to occur during grape ripening. Probes for homogalacturonan (e.g. LM19) were enriched in the CDTA fractions of Crimson Seedless relative
to Cabernet Sauvignon grapes. Probes for pectic-β-(1,4)-galactan (mAb LM5), extensin (mAb LM1) and arabinogalactan proteins (AGPs, mAb LM2) were strongly correlated with ripening. From green stage to veraison, a progressive reduction in pectic-β-(1,4)-galactan epitopes, present in both pectin-rich (CDTA) and hemicelluloserich (NaOH) polymers, was observed. Ripening changes in AGP and extensin epitope abundance also were found during and after veraison.

†Conclusions:
Combinations of cell wall probes are able to define distinct ripening phases in grapes. Pectic-β-(1,4)-
galactan epitopes decreased in abundance from green stage tove´raison berries. Fromve´raison therewas an increase in abundance of significant extensin andAGPepitopes, which correlates with cell expansion events. This study provides new ripening biomarkers and changes that can be placed in the context of grape berry development.

Key words: Profiling, berry ripening, plant cell wall, pectic-β-(1,4)-galactan, extensin, arabinogalactan–protein, AGP, wine grapes, table grapes, Vitis vinifera, Cabernet Sauvignon, Crimson Seedless.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2014

Deskripsi Fisik

Annals of Botany 114: 1279–1294, 2014

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

FISIOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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