e-journal

Plastid DNA sequencing and nuclear SNP genotyping help resolve the puzzle of central American Platanus

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† Background and Aims:
Recent research on the history of Platanus reveals that hybridization phenomena occurred in the central American species. This study has two goals: to help resolve the evolutive puzzle of central American
Platanus, and to test the potential of real-time polymerase chain reaction (PCR) for detecting ancient hybridization.

† Methods:
Sequencing of a uniparental plastidDNAmarker [ psbA-trnH(GUG) intergenic spacer] and qualitative and
quantitative single nucleotide polymorphism (SNP) genotyping of biparental nuclear ribosomal DNA (nrDNA)
markers [LEAFY intron 2 (LFY-i2) and internal transcribed spacer 2 (ITS2)] were used.

† Key Results:
Based on the SNP genotyping results, several Platanus accessions showthe presence of hybridization/ introgression, including some accessions ofP. rzedowskii and ofP.mexicana var. interior and one ofP. mexicana var. mexicana fromOaxaca (¼ P. oaxacana). Based on haplotype analyses of the psbA-trnH spacer, five haplotypeswere detected. The most common of these is present in taxa belonging toP. orientalis,P. racemosa sensu lato, some accessions ofP. occidentalis sensu stricto (s.s.) fromTexas,P. occidentalis var. palmeri,P.mexicana s.s. andP. rzedowskii. This is highly relevant to genetic relationships with the haplotypes present in P. occidentalis s.s. andP. mexicana var. interior.

†Conclusions:
Hybridization and introgression events between lineages ancestral to moderncentral and eastern North
American Platanus species occurred. Plastid haplotypes and qualitative and quantitative SNP genotyping provide information critical for understanding the complex history ofMexican Platanus.Compared with the usual molecular techniques of sub-cloning, sequencing and genotyping, real-time PCR assay is a quick and sensitive technique for analysing complex evolutionary patterns.

Key words: Central America, genotyping, hybridization, introgression, internal transcribed spacer 2, LEAFY intron 2, Platanus, psbA-trnH(GUG) intergenic spacer, real-time PCR, sequencing, SNP.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2013

Deskripsi Fisik

Annals of Botany 112: 589–602, 2013

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

112: 2013

Subjek

FISIOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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