e-journal

The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley

---

† Background and Aims:
Aluminium is toxic in acid soils because the soluble Al3+ inhibits root growth.Amechanism of Al3+ tolerance discovered in many plant species involves the release of organic anions fromroot apices. The Al3+ -
activated release of citrate from the root apices of Al3+ -tolerant genotypes of barley is controlled by a MATE gene named HvAACT1 that encodes a citrate transport protein located on the plasma membrane. The aim of this study was to investigate whether expressing HvAACT1 with a constitutive promoter in barley and wheat can increase citrate efflux and Al3+ tolerance of these important cereal species.

† Methods:
HvAACT1 was over-expressed in wheat (Triticum aestivum) and barley (Hordeum vulgare) using the maize ubiquitin promoter. Root apices of transgenic and control lines were analysed for HvAACT1 expression and organic acid efflux. The Al3+ tolerance of transgenic and control lines was assessed in both hydroponic solution and acid soil.

† Key Results and Conclusions:
Increased HvAACT1 expression in both cereal species was associated with increased citrate efflux from root apices and enhanced Al3+ tolerance, thus demonstrating that biotechnology can complement traditional breeding practices to increase the Al3+ tolerance of important crop plants.

Key words: Acid soil, aluminium, resistance, tolerance, wheat, transgenic, barley, Hordeum vulgare, Triticum
aestivum.

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

-

Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2013

Deskripsi Fisik

Annals of Botany 112: 603–612, 2013

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

112: 2013

Subjek

EKOFISIOLOGI TANAMAN

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar