e-journal

Functional study of dextran-graft-poly((2-dimethyl amino)ethyl methacrylate) gene delivery vector for tumor therapy

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The obstacle of gene therapy is the shortage of efficient delivery system. The development of the gene delivery system with high transfection efficiency and low toxicity appears to be crucial. Recently, we reported that the extrangraft-
poly((2-dimethyl amino)ethyl methacrylate) (DPD) can be potentially used as efficient gene vector. Herein, DPD was systematically studied for its potential in tumor gene therapy. DPD was synthesized and characterized by agarose gel electrophoresis, particle size and zeta potential. The particle size and zeta potential of the DPD/enhanced green fluorescent protein (pEGFP-C1) plasmid complexes at various N/P ratios were 130–150 nm and about 40mV, respectively.The results showed that DPD exhibit a higher transfection effect compared with Lipofectamine 2K (Lipo 2K), a commercialized
vector. The possibility of DPD in gene therapy was evaluated using p53, a gene that has been wildly applied in the research of cancer gene therapy. DPD/pEGFP-C1–p53 complex was found to be able to inhibit tumor cell proliferation
through cell cycle arrest and apoptosis. Moreover, the tumor growth was found to be restrained when DPD/ pEGFP-C1–p53 complex was used in a xenograft MCF7 tumor model in vivo. These observations indicated that DPD/pEGFP-C1–p53 complex may be considered to be an efficient delivery system for tumor gene therapy.

Keywords DPD, pEGFP-C1–p53, tumor suppression, gene therapy

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Informasi Detail

Judul Seri

Journal of Biomaterials Applications

No. Panggil

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Penerbit

: SAGE Publications Ltd., 2012

Deskripsi Fisik

Journal of Biomaterials Applications 28(1) 125–135

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOKIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

hasnidar/agus

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