e-journal

Molecular weight-dependent genetic information transfer with disulfide-linked polyethylenimine-based nonviral vectors

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One strategy for improving gene vector properties of polyethylenimine is to facilitate individual transfection mechanism steps. This study investigates (i) improving transfection efficiency by attaching peptide nuclear localization signals (nuclear localization signals: SV40 large T antigen nuclear localization signal or C-terminus of histone H1) to polyethylenimine (10 kDa) and (ii) using disulfide linkages, which are expected to be stable during polyplex formation, but cleaved inside
cells giving improved gene release. Nuclear localization signal-containing polyplexes exhibited low cytotoxicity, whereas
transfection efficiency with high molecular weight plasmid DNA increased up to 3.6 times that of underivatized polyethylenimine in Neuro2A cells at higher molar ratio of polyethylenimine-nitrogen to DNA-phosphate (N/P) ratios. However, with luciferase-specific low molecular weight small interfering RNA in Neuro2A/EGFPLuc cells, nuclear localization signal-containing polyplexes with disulfide linkages caused substantial cytotoxicity at N/P ratios >15 and no consistent significant reduction in luciferase expression. Possible explanations for molecular weight-dependent differences
in genetic information transfer by polyplexes containing disulfide-linked nuclear localization signals are discussed.

Keywords Gene therapy, siRNA, histone H1 C-terminal tail, nuclear localization signal, polyethylenimine

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Informasi Detail

Judul Seri

Journal of Biomaterials Applications

No. Panggil

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Penerbit

: SAGE Publications Ltd., 2012

Deskripsi Fisik

Journal of Biomaterials Applications 28(1) 112–124

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOKIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

hasnidar/agus

Versi lain/terkait

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