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ARAG1, an ABA-responsive DREB gene, plays a role in seed germination and drought tolerance of rice

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Background and Aims DREB proteins are involved mainly in plant responses to abiotic stresses such as cold,
drought or high salinity as well as ABA signalling. However, the function of most rice DREB genes and the
underlying molecular mechanisms controlling these responses remains elusive. In this study, ARAG1, a rice
DREB gene, was functionally analysed.
Methods Antisense and over-expression constructs of ARAG1 were introduced into rice by an Agrobacteriummediated method. RT-PCR and western blot were used to detect ARAG1 accumulation in transgenics. PEG and ABA were used to test their response to abiotic stresses.
Key Results ARAG1 was expressed in inflorescences, roots, immature embryos and germinating seeds, but not in coleoptiles, leaves or mature embryos. Drought stress and ABA treatment increased transcript levels of the gene rapidly. ARAG1 knockdown line was hypersensitive to ABA application during seed germination and seedling growth. However, the line over-expressing ARAG1 behaved similarly to wild type in these circumstances. Knockdown of ARAG1 weakened tolerance of the transgenic seedlings to drought stress, while over-expression of it increased the tolerance slightly. In addition, activity of a-amylases was enhanced in germinating seeds of the knockdown and over-expression lines.
Conclusions These results indicate that ARAG1 was involved in the ABA signalling and stress responsive
pathways.
Key words: Abscisic acid (ABA), AP2/EREBP, ARAG1, DREB, drought, Oryza sativa.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2010

Deskripsi Fisik

Annals of Botany 105: 401–409, 2010

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

Versi lain/terkait

Lampiran Berkas

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