e-journal

Role of macrophage activation in the lipid metabolism of postprandial triacylglycerol-rich lipoproteins

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The potential link between the inflammatory effects of postprandial lipemia and the induction of macrophage foam cell
formation by triacylglycerol-rich lipoproteins (TGRL) was studied using postprandial triacylglycerol-rich lipoproteins
(ppTGRL) derived from human volunteers and primary human monocyte-derived macrophages (HMDM). Subjects were fed
a test meal high in dairy fat, followed three hours later by isolation of serum ppTGRL. Pro-inflammatory (M1) and antiinflammatory (M2) phenotypes were induced in HMDM by treatment with lipopolysaccharide (LPS) or dexamethasone
(DEX), respectively. ppTGRL caused a dose-dependent increase in both triacylglycerol (TG) and cholesterol (CH)
accumulation in the cells. TG accumulation was unaffected by LPS or DEX treatment, but LPS as compared with DEXtreated
HMDM were found to accumulate more CH, and this effect was greater than that induced by ppTGRL in untreated
cells. LPS-treatment had no effect on lipid uptake from ppTGRL (via the LDLr, scavenger receptors or SR-B1) or on CH
efflux, but the CH synthesis inhibitor mevinolin abolished the difference between CH accumulation in LPS-and DEX-treated
cells, suggesting that CH synthesis is enhanced in the inflammatory state. Phospholipid (PL) synthesis was increased in
inflammatory M1 as compared with anti-inflammatory M2 HMDM. Moreover, TG synthesis was decreased by ppTGRL in
DEX-treated as compared with untreated cells. We conclude, therefore, inflammation causes a greater increase in the
accumulation of neutral lipids than ppTGRL in macrophages, and that this effect is related to modulation of PL metabolism
and possibly also CH synthesis. Thus, the inflammatory phenotype of macrophages influences their lipid metabolism, and
is, therefore, likely to modulate the induction of macrophage lipid accumulation by lipoproteins associated with foam cell formation.

Keywords: human monocyte-derived macrophages, macrophage phenotype, postprandial triacylglycerol-rich lipoproteins,
lipid metabolism

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Informasi Detail

Judul Seri

Experimental Biology and Medicine

No. Panggil

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Penerbit

: SAGE Publications Ltd., 2013

Deskripsi Fisik

Experimental Biology and Medicine 2013; 238: 98–110

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOKIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

hasnidar/agus

Versi lain/terkait

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