e-journal

The spatial expression and regulation of transcription factors IDEF1 and IDEF2

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Background and Aims Under conditions of low iron availability, rice plants induce genes involved in iron
uptake and utilization. The iron deficiency-responsive cis-acting element binding factors 1 and 2 (IDEF1 and IDEF2) regulate transcriptional response to iron deficiency in rice roots. Clarification of the functions of IDEF1 and IDEF2 could uncover the gene regulation mechanism.
Methods Spatial patterns of IDEF1 and IDEF2 expression were analysed by histochemical staining of IDEF1
and IDEF2 promoter-GUS transgenic rice lines. Expression patterns of the target genes of IDEF1 and IDEF2
were analysed using transformants with induced or repressed expression of IDEF1 or IDEF2 grown in ironrich
or in iron-deficient solutions for 1 d.
Key Results IDEF1 and IDEF2 were highly expressed in the basal parts of the lateral roots and vascular
bundles. IDEF1 and IDEF2 expression was dominant in leaf mesophyll and vascular cells, respectively. These
expression patterns were similar under both iron-deficient and iron-sufficient conditions. IDEF1 was strongly expressed in pollen, ovaries, the aleurone layer and embryo. IDEF2 was expressed in pollen, ovaries and the dorsal vascular region of the endosperm. During seed germination, IDEF1 and IDEF2 were expressed in the endosperm and embryo. Expression of IDEF1 target genes was regulated in iron-rich roots similar to early iron-deficiency stages. In addition, the expression patterns of IDEF2 target genes were similar between ironrich conditions and early or subsequent iron deficiency.
Conclusions IDEF1 and IDEF2 are constitutively expressed during both vegetative and reproductive stages. The spatial expression patterns of IDEF1 and IDEF2 overlap with their target genes in restricted cell types, but not in all cells. The spatial expression patterns and gene regulation of IDEF1 and IDEF2 in roots are generally conserved under conditions of iron sufficiency and deficiency, suggesting complicated interactions with unknown factors for sensing and transmitting iron-deficiency signals.
Key words: IDE, IDEF, iron deficiency, Oryza sativa, reproductive organs, seeds, transcriptional regulation, vegetative organs.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2010

Deskripsi Fisik

Annals of Botany 105: 1109–1117, 2010

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

Versi lain/terkait

Lampiran Berkas

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