e-journal

Alternate transcripts of a floral developmental regulator have both distinct and redundant functions in opium poppy

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Background and Aims The MADS-box transcription factor AGAMOUS (AG) is an important regulator of
stamen and fruit identity as well as floral meristem determinacy in a number of core eudicots and monocots. However, its role outside of these groups has not been assessed explicitly. Examining its role in opium poppy, a basal eudicot, could uncover much about the evolution and development of flower and fruit development in the angiosperms.
Methods AG orthologues were isolated by degenerate RT-PCR and the gene sequence and structure examined;
gene expression was characterized using in situ hybridization and the function assessed using virus-induced gene silencing.
Key Results In opium poppy, a basal eudicot, the AGAMOUS orthologue is alternatively spliced to produce
encoded products that vary at the C-terminus, termed PapsAG-1 and PapsAG-2. Both transcripts are expressed
at high levels in stamens and carpels. The functional implications of this alternative transcription were examined using virus-induced gene silencing and the results show that PapsAG-1 has roles in stamen and carpel identity, reflecting those found for Arabidopsis AG. In contrast, PapsAG-2, while displaying redundancy in these functions, has a distinctive role in aspects of carpel development reflected in septae, ovule and stigma defects seen in the loss-of-function line generated.
Conclusions These results describe the first explicit functional analysis of an AG-clade gene in a basal eudicot; illustrate one of the few examples of the functional consequences of alternative splicing in transcription factors and reveal the importance of alternative transcription, as well as gene duplication, as a driving force in evolution.
Key words: Flower development, MADS-box, Papaver somniferum, alternative transcription, AGAMOUS,
gene-silencing.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 107: 1557–1566, 2011

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

Versi lain/terkait

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