e-journal

Analyses of two rice (Oryza sativa) cyclin-dependent kinase inhibitors and effects of transgenic expression of OsiICK6 on plant growth and development

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Background and Aims Plants have a family of proteins referred to as ICKs (inhibitors of cyclin-dependent
kinase, CDK) or KRPs (Kip-related proteins) that function to regulate the activities of CDK. Knowledge of
these plant CDK inhibitors has been gained mostly from studies of selected members in dicotyledonous
plants, particularly Arabidopsis. Much remains to be learned regarding the differences among various
members of the ICK/KRP family, and regarding the function and regulation of these proteins in monocotyledonous plants.
MethodsWe analysed ICK-related sequences in the rice (Orysa sativa L. subsp. indica) genome and determined
that there are six members with the conserved C-terminal signature region for ICK/KRP proteins. They are
referred to as OsiICKs and further analyses were performed. The interactions with CDKs and cyclins were determined by a yeast two-hybrid assay, and cellular localization by fusion with the enhanced green fluorescence protein (EGFP). The expression of OsiICK6 in different tissues and in response to several treatments was analysed by reverse transcriptase-mediated polymerase chain reaction (RT-PCR) and real-time PCR. Furthermore, OsiICK6 was over-expressed in transgenic rice plants and significant phenotypes were observed.
Key Results and Conclusions Based on putative protein sequences, the six OsiICKs are grouped into two
classes, with OsiICK1 and OsiICK6 in each of the two classes, respectively. Results showed that OsiICK1
and OsiICK6 interacted with OsCYCD, but differed in their interactions with CDKA. Both EGFP:OsiICK1
and EGFP:OsiICK6 were localized in the nucleus. Whereas EGFP:OsiICK6 showed a punctuate subnuclear distribution, OsiICK1 had a homogeneous pattern. Over-expression of OsiICK6 resulted in multiple phenotypic effects on plant growth, morphology, pollen viability and seed setting. In OsiICK6-over-expressing plants, leaves rolled toward the abaxial side, suggesting that cell proliferation is critical in maintaining an even growth along the dorsal–ventral plane of leaf blades.
Key words: Cell cycle, cyclin-dependent kinase inhibitor, leaf curvature, ICK, nuclear localization,
Oryza sativa, plant growth, protein–protein interaction, rice, seed yield, stress-induced expression.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 107: 1087–1101, 2011

Bahasa

English

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

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