e-journal

Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress

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Background and Aims In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana;CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested.
Methods arath;cdc25 T-DNA insertion lines, Arath;CDC25 over-expressing lines and wild type were challenged
with hydroxyurea (HU) and zeocin, substances that stall DNA replication and damage DNA, respectively,
together with an abiotic stressor, NaCl. A molecular and phenotypic assessment was made of all genotypes
Key Results There was a null phenotypic response to perturbation of Arath;CDC25 expression under control
conditions. However, compared with wild type, the arath;cdc25 T-DNA insertion lines were hypersensitive to
HU, whereas the Arath;CDC25 over-expressing lines were relatively insensitive. In particular, the over-expressing lines consistently outgrew the T-DNA insertion lines and wild type when challenged with HU. All genotypes were equally sensitive to zeocin and NaCl.
Conclusions Arath;CDC25 plays a role in overcoming stress imposed by HU, an agent know to induce the DNA
replication checkpoint in arabidopsis. However, it could not enhance tolerance to either a zeocin treatment, known to induce DNA damage, or salinity stress.
Key words: Arabidopsis thaliana, cell-cycle checkpoints, hydroxyurea, root growth, NaCl, zeocin.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 107: 1183–1192, 2011

Bahasa

English

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

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yuli/agus

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