e-journal

Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches

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Background and Aims The oomycete Aphanomyces euteiches causes up to 80 % crop loss in pea (Pisum
sativum). Aphanomyces euteiches invades the root system leading to a complete arrest of root growth and ultimately to plant death. To date, disease control measures are limited to crop rotation and no resistant pea lines are available. The present study aims to get a deeper understanding of the early oomycete–plant interaction at the tissue and cellular levels.
Methods Here, the process of root infection by A. euteiches on pea is investigated using flow cytometry and microscopic techniques. Dynamic changes in secondary metabolism are analysed with high-performance
liquid chromatography with diode-array detection.
Key Results Root infection is initiated in the elongation zone but not in the root cap and border cells. Bordercell production is significantly enhanced in response to root inoculation with changes in their size and morphology. The stimulatory effect of A. euteiches on border-cell production is dependent on the number of oospores inoculated. Interestingly, border cells respond to pathogen challenge by increasing the synthesis of the phytoalexin pisatin.
Conclusions Distinctive responses to A. euteiches inoculation occur at the root tissue level. The findings suggest that root border cells in pea are involved in local defence of the root tip against A. euteiches. Root border cells constitute a convenient quantitative model to measure the molecular and cellular basis of plant–microbe interactions.
Key words: Aphanomyces euteiches, pathogen, phenolics, Pisum sativum, pisatin, root border cells, root cap, root infection.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 108: 459–469, 2011

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

Versi lain/terkait

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