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Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters

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Background and Aims Brachypodium distachyon is a temperate grass with a small stature, rapid life cycle and completely sequenced genome that has great promise as a model system to study grass-specific traits for crop improvement. Under iron (Fe)-deficient conditions, grasses synthesize and secrete Fe(III)-chelating agents called phytosiderophores (PS). In Zea mays, Yellow Stripe1 (ZmYS1) is the transporter responsible for the uptake of Fe(III)–PS complexes from the soil. Some members of the family of related proteins called Yellow Stripe-Like (YSL) have roles in internal Fe translocation of plants, while the function of other members remains uninvestigated. The aim of this study is to establish brachypodium as a model system to study Fe homeostasis in grasses, identify YSL proteins in brachypodium and maize, and analyse their expression profiles in brachypodium in response to Fe deficiency.
Methods The YSL family of proteins in brachypodium and maize were identified based on sequence similarity
to ZmYS1. Expression patterns of the brachypodium YSL genes (BdYSL genes) were determined by quantitative
RT–PCR under Fe-deficient and Fe-sufficient conditions. The types of PS secreted, and secretion pattern of PS in brachypodium were analysed by high-performance liquid chromatography.
Key Results Eighteen YSL family members in maize and 19 members in brachypodium were identified.
Phylogenetic analysis revealed that some YSLs group into a grass-specific clade. The Fe status of the plant can regulate expression of brachypodium YSL genes in both shoots and roots. 3-Hydroxy-2′-deoxymugineic acid (HDMA) is the dominant type of PS secreted by brachypodium, and its secretion is diurnally regulated.
Conclusions PS secretion by brachypodium parallels that of related crop species such as barley and wheat.
A single grass species-specific YSL clade is present, and expression of the BdYSL members of this clade
could not be detected in shoots or roots, suggesting grass-specific functions in reproductive tissues. Finally, the Fe-responsive expression profiles of several YSLs suggest roles in Fe homeostasis.
Key words: Brachypodium distachyon, Zea mays, iron homeostasis, phytosiderophore, nicotianamine, Yellow
Stripe-Like, YSL, YS1.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 108: 821–833, 2011

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

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