e-journal

Identification of the Protein Target of Myelin-Binding Ligands by Immunohistochemistry and Biochemical Analyses

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The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic protein (MBP) that provides quantitative evidence that MBP is the molecular binding partner of previously described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111. (J Histochem Cytochem 61:19–30, 2013)

Keywords fluorescence polarization, myelin basic protein, nerves, myelin, dye

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Informasi Detail

Judul Seri

Journal of Histochemistry & Cytochemistry

No. Panggil

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Penerbit

: SAGE Publications Ltd., 2013

Deskripsi Fisik

Journal of Histochemistry & Cytochemistry 61(1) 19– 30 , 2013

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOKIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

hasnidar/agus

Versi lain/terkait

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