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Accurate Quantitation of Ki67-positive Proliferating Hepatocytes in Rabbit Liver by a Multicolor Immunohistochemical (IHC) Approach Analyzed with Automated Tissue and Cell Segmentation Software

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Determination of hepatocyte proliferation activity is hampered by the presence of Ki67-positive non-parenchymal cells.
We validated a multicolor immunohistochemical (IHC) approach using multispectral tissue and cell segmentation software.
Portal vein branches to the cranial liver lobes of 10 rabbits were embolized, leading to atrophy of the cranial lobes and
hyperplasia of the caudal lobes. Slides from cranial and caudal lobes (n=20) were double-stained (CK8+18 and Ki67) and
triple-stained (CK8+18, Ki67, and CD31). The Ki67 proliferation index was calculated using automated tissue and cell
segmentation software and compared with manual counting by two independent observers. A substantial variation was
seen in the number of Ki67-positive hepatocytes in the different specimens in both double and triple staining (range, 0–50). Correlation coefficients between manual counting and the digital analysis were 0.76 for observer 1 (p

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Informasi Detail

Judul Seri

Journal of Histochemistry & Cytochemistry

No. Panggil

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Penerbit

: SAGE Publications Ltd., 2013

Deskripsi Fisik

Journal of Histochemistry & Cytochemistry 61(1) 11– 18 , 2013

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOKIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

hasnidar/agus

Versi lain/terkait

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