e-journal

Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus

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Background and Aims Catharanthus roseus is a plant of great medicinal importance, yet inadequate knowledge
of its genome structure and the unavailability of genomic resources have been major impediments in the development of improved varieties. The aims of this study were to develop co-dominant sequence-tagged microsatellite sites (STMS) and gene-targeted markers (GTMs) and utilize them for the construction of a framework intraspecific linkage map of C. roseus.
Methods For simple sequence repeat (SSR) isolation, a genomic library enriched for (GA)n repeats was
constructed from C. roseus ‘Nirmal’ (CrN1). In addition, GTMs were also designed from 12 genes of the TIA
(terpenoid indole alkaloid) pathway – the medicinally most significant pathway in C. roseus. An F2 mapping
population was also generated by crossing two diverse accessions of C. roseus CrN1 (Nirmal)×CrN82 (Kew).
Key Results A new set of 314 STMS markers and 64 GTMs were developed in this study. A segregating F2
mapping population consisting of 111 F2 individuals was generated. For generating the linkage map, a set of 423 co-dominant markers (378 newly developed and 45 published earlier) were screened for polymorphism
between the parental genotypes, of which 134 were identified to be polymorphic. A total of 114 markers were mapped on eight linkage groups that spanned a 632.7 cM region of the genome with an average marker distance of 5.55 cM. Further, the mechanism of hypervariability at the gene-targeted loci was investigated at the sequence level.
Conclusions For the first time, a large array of STMS markers and GTMs was generated in the model medicinal plant C. roseus. Moreover, the first microsatellite marker-based linkage map was described in this study. Together, these will serve as a foundation for future genomics studies related to quantitative trait loci analysis and molecular breeding in C. roseus.
Key words: Catharanthus roseus, sequence-tagged microsatellite sites, STMS, microsatellites, linkage map,
gene-targeted markers, GTM.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

: Oxford University Press on behalf of the Annals of Botany Company., 2011

Deskripsi Fisik

Annals of Botany 108: 321–336, 2011

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

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