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DNA barcoding: a new tool for palm taxonomists?

Marc L. Jeanson - Nama Orang; Jean-Noe¨l Labat - Nama Orang; Damon P. Little - Nama Orang;

Background and Aims In the last decade, a new tool – DNA barcoding – was proposed to identify species. The
technique of DNA barcoding is still being developed. The Consortium for the Barcode of Life’s Plant Working Group (CBOL-PWG) selected two core markers (matK and rbcL) that now must be tested in as many taxa as possible. Although the taxonomy of palms (Arecaceae/Palmae) has been greatly improved in the past decades, taxonomic problems remain. Species complexes, for example, could significantly benefit from DNA barcoding. Palms have never before been subjected to a DNA barcoding test.
Methods For this study, 40 out of the 48 species of the southeast Asian tribe Caryoteae (subfamily
Coryphoideae) were included. In total, four DNA markers – three plastid encoded (matK, rbcL and psbAtrnH)
and one nuclear encoded (nrITS2) – were analysed to determine if adequate variation exists to discriminate
among species.
Keys Results The combination of three markers – matK, rbcL and nrITS2 – results in 92% species discrimination.
This rate is high for a barcoding experiment. The two core markers suggested by the CBOL-PWG,
rbcL and matK, have a low species discrimination rate and need to be supplemented by another marker. In
Caryoteae, nrITS2 should be chosen over psbA-trnH to supplement the two ‘core’ markers.
Conclusions For the first time a test of DNA barcoding was conducted in Arecaceae. Considering that palms
have highly variable mutation rates compared with other angiosperms, the results presented here are encouraging for developing DNA barcoding as a useful tool to identify species within this ecologically important tropical plant family.
Key words: DNA barcoding, Arecaceae, Caryoteae, Caryota, Arenga, Wallichia, rbcL, matK, psbA-trnH,
nrITS2.

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Judul Seri: Annals of Botany
No. Panggil: -
Penerbit: : Oxford University Press on behalf of the Annals of Botany Company., 2011
Deskripsi Fisik: Annals of Botany 108: 1445–1451, 2011
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: PERTANIAN.
Info Detail Spesifik: -
Pernyataan Tanggungjawab: yuli/agus

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DNA barcoding: a new tool for palm taxonomists?

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