e-book

Environmental Microbiology: Methods and Protocols

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The methods included in Environmental Microbiology: Methods and Protocols
can be placed in the categories “Communities and Biofilms,” “Fermented
Milks,” “Recovery and Determination of Nucleic Acids,” and the review section,
containing chapters on the endophytic bacterium, Bacillus mojavensis,
the engineering of bacteria to enhance their ability to carry out bioremediation
of aromatic compounds, using the hemoglobin gene from a strain of Vitreoscilla
spp., and the use of chemical shift reagents and 23Na NMR to study sodium
gradients in microorganisms, all of which should be of interest to investigators
in these fields.

The subjects treated within the different categories also cover a wide range,
with methods ranging from those for the study of marine organisms, through
those for the investigation of microorganisms occurring in ground waters,
including subsurface ground waters, to other types of environmental waters, to
as varied subjects as the biodiversity of yeasts found in northwest Argentina.
The range of topics described in the Fermented Milks section is smaller, but
significant for investigators in areas concerned with milk as an item of foods
for infants, small children, and even adults.

The section on recovery and determination of nucleic acids and other compounds
affecting, and affected by, microorganisms also covers a considerable
range, as well as including methods for some of the enzymes produced by plant
pathogens and methods for obtaining microbial species tolerant of some
inhibitors, such as heavy metals. Thus, there is something for most investigators
concerned with microorganisms in their native environments.

There is one aspect of Environmental Microbiology: Methods and Protocols
concerned with the special problem of microorganisms in the environment,
that of the so-called “non-culturable” forms. This problem has been
solved, at least partially, by growing the organisms in a two-chambered system
in which the organisms of interest are grown in a central chamber, separated by
a semipermeable membrane from an outer compartment, in which a mixed
culture of an unidentified group of organisms is grown, and produces unidentified
(as yet) growth factors for the organisms in the central chamber. This problem
has existed for at least 50 years, for soil microbiologists and probably
others. The method is described in a recent issue of Science. Unfortunately, the
editors were unable to find an author willing, or able to take the time, to write
about this subject.

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Ketersediaan

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Informasi Detail

Judul Seri

Methods in biotechnology ; 16

No. Panggil

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Penerbit

New Jersey : Humana Press Inc.., 2004

Deskripsi Fisik

QR100.E584; 579--dc22 2004008392

Bahasa

English

ISBN/ISSN

1-58829-116-2

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

MIKROBIOLOGI
MIKROBIOLOGI LINGKUNGAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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